"Reliability of t7-based mRNA linear amplification validated by gene expression analysis of human kidney cells using cDNA microarrays.”

Tables

Table 1.

Pearson coefficient r for intra-sample variability (reproducibility).

Table 2.

Pearson coefficient r for inter-sample variability (linearity).

Table 3a.

Gene expression values of selected genes before and after linear amplification.

Table 3b.

Gene expression values of selected genes before and after linear amplification.

Table 4.

Comparison of microarray results with results from qRT-PCR.
Figures

Figure 1a.

Scatter plots of intra-sample comparisons (reproducibility testing): unamplified sample 1 vs. 2.

Figure 1b.

Scatter plots of intra-sample comparisons (reproducibility testing): one round amplified RNA 1 vs. 2.

Figure 1c.

Scatter plots of intra-sample comparisons (reproducibility testing): 2nd round amplified RNA 1 vs. 2.

Figure 1d.

Scatter plots of intra-sample comparisons (reproducibility testing): 2 successive rounds of amplification 1 vs. 2.

Figure 2a.

Selected scatter plots of inter-sample comparisons (linearity testing): unamplified sample 1 vs. one round amplified sample 2.

Figure 2b.

Selected scatter plots of inter-sample comparisons (linearity testing): unamplified sample 2 vs. 2nd round amplified sample 2.

Figure 2c.

Selected scatter plots of inter-sample comparisons (linearity testing): unamplified sample 3 vs. 2 successive rounds of amplification sample 3.
Impressum
Dr. Michael Rudnicki
Medical University Innsbruck
Department of Internal Medicine IV, Nephrology und Hypertension
Anichstrasse 35, A-6020 Innsbruck
Tel: +43 512 504 81337, Fax: +43 512 504 25857
michael.rudnicki@i-med.ac.at