"In vitro-transcriptional response of polymorphonuclear leukocytes following contact with different antigens.”

Tables

Table 1.

List of the most differentially regulated genes between complete and "frustrated" phagocytosis. The fold increase of the most differentially regulated genes between PMN stimulated with E.coli as compared to HD membrane stimulated PMN is shown.

Webtable 1.

Microarray raw data list of 50 differentially regulated genes between controls and HD-membrane-stimulated PMNs.

Webtable 2.

Microarray raw data list of 88 differentially regulated genes between controls and E.coli-stimulated PMNs.

Webtable 3.

Microarray raw data list of 53 genes being differentially regulated during complete and incomplete phagocytosis.
Figures

Figure 1.

Supervised cluster analysis of unstimulated (UN), cuprophane-(CU), polysulfone- (PS) and E.coli-stimulated PMNs.

Figure 2.

Significantly differentially regulated genes in HD-membrane-stimulated PMN. Data are presented as fold increase in gene expression of respective genes. Dark green box < -5fold, green box -5 to -3fold, light green box -3 to 0fold, light red box 0 to 3fold, orange box 3 to 5fold, red box > 5fold gene expression in CU/PS-stimulated compared to unstimulated PMN.

Figure 3.

Significantly differentially regulated genes in E.coli stimulated PMN. Data are presented as fold increase in gene expression of respective genes. Dark green box < -5fold, green box -5 to -3fold, light green box -3 to 0fold, light red box 0 to 3fold, orange box 3 to 5fold, red box > 5fold gene expression in E.coli-stimulated compared to unstimulated PMN.

Figure 4.

Comparison between data from quantitative PCR and microarrays. On one hand quantitative PCR for detection of FADD, SOCS3, IL1B, JUN and FOS was performed in not amplificated cDNA samples from CU-stimulated PMNs and unstimulated PMNs. On the other hand, expression of the respective genes from microarray experiments was evaluated. Differences in translation of the respective genes between CU-stimulated PMNs and unstimulated PMNs are provided. The Pearson R is provided in the diagram.

Figure 5.

Protein expression of IL-1 beta. PMNs of healthy donors were stimulated for one hour with CU or with PBS (UN) in the presence of human serum. Western blots for the detection of IL-1β were performed. Afterwards, blots were reblotted for detection of β-actin as a loading control. 2 independent experiments are shown.
Impressum
Dr. Michael Rudnicki
Medical University Innsbruck
Department of Internal Medicine IV, Nephrology und Hypertension
Anichstrasse 35, A-6020 Innsbruck
Tel: +43 512 504 81337, Fax: +43 512 504 25857
michael.rudnicki@i-med.ac.at